diff --git a/IRaCIS.Core.Application/Service/Reading/ClinicalData/ClinicalQuestionService.cs b/IRaCIS.Core.Application/Service/Reading/ClinicalData/ClinicalQuestionService.cs
index 93b92894a..95220eb33 100644
--- a/IRaCIS.Core.Application/Service/Reading/ClinicalData/ClinicalQuestionService.cs
+++ b/IRaCIS.Core.Application/Service/Reading/ClinicalData/ClinicalQuestionService.cs
@@ -233,6 +233,11 @@ namespace IRaCIS.Core.Application.Service
                 return ResponseOutput.NotOk(_localizer["ClinicalQuestion_Repeat"]);
             }
 
+            if (await _trialClinicalQuestionRepository.AnyAsync(x => x.TrialClinicalId == inDto.TrialClinicalId && x.Id != inDto.Id && x.ShowOrder == inDto.ShowOrder))
+            {
+                return ResponseOutput.NotOk("问题序号存在重复！");
+            }
+
             var entity = await _trialClinicalQuestionRepository.InsertOrUpdateAsync(inDto, true);
             return ResponseOutput.Ok(entity.Id.ToString());
 
@@ -315,6 +320,11 @@ namespace IRaCIS.Core.Application.Service
             {
                 return ResponseOutput.NotOk(_localizer["ClinicalQuestion_Repeat"]);
             }
+
+            if (await _systemClinicalQuestionRepository.AnyAsync(x => x.SystemClinicalId == inDto.SystemClinicalId && x.Id != inDto.Id && x.ShowOrder == inDto.ShowOrder))
+            {
+                return ResponseOutput.NotOk("问题序号存在重复！");
+            }
             var entity = await _systemClinicalQuestionRepository.InsertOrUpdateAsync(inDto, true);
             return ResponseOutput.Ok(entity.Id.ToString());
 
@@ -401,6 +411,11 @@ namespace IRaCIS.Core.Application.Service
                 return ResponseOutput.NotOk(_localizer["ClinicalQuestion_Repeat"]);
             }
 
+            if (await _systemClinicalTableQuestionRepository.AnyAsync(x => x.QuestionId == inDto.QuestionId && x.Id != inDto.Id && x.ShowOrder == inDto.ShowOrder))
+            {
+                return ResponseOutput.NotOk("问题序号存在重复!");
+            }
+
             var entity = await _systemClinicalTableQuestionRepository.InsertOrUpdateAsync(inDto, true);
 
             return ResponseOutput.Ok(entity.Id.ToString());
@@ -455,6 +470,11 @@ namespace IRaCIS.Core.Application.Service
             {
                 return ResponseOutput.NotOk(_localizer["ClinicalQuestion_Repeat"]);
             }
+
+            if (await _trialClinicalTableQuestionRepository.AnyAsync(x => x.QuestionId == inDto.QuestionId && x.Id != inDto.Id && x.ShowOrder == inDto.ShowOrder))
+            {
+                return ResponseOutput.NotOk("问题序号存在重复!");
+            }
             var entity = await _trialClinicalTableQuestionRepository.InsertOrUpdateAsync(inDto, true);
 
             return ResponseOutput.Ok(entity.Id.ToString());